Dirk Berressem, Ph.D.

162180915D4S 7074B

Dirk Berressem holds a Master of Science (M.Sc.) in chemistry and is currently worked on the project entitled "long-chain polyunsaturated fatty acids in support of intervention and rehabilitation of ischemic stroke".  To elaborate this project, he applied several in vivo and in vitro methods to measure mitochondrial dysfunction, synaptic plasticity and impact of stroke to neuronal cells. He received his Ph.D. in April 2017.

Methods:        

  • Measurement of mitochondrial membrane potential (MMP) and determination of Adenosine-triphosphat (ATP) levels
  • High-Resolution Respirometry (Oxygraph-2k)
  • Western Blot
  •  Gas chromatography-mass spectrometry (GC-MS)
  • High-performance liquid chromatography (HPLC)
  • Animal studies
  • Surgery - Applying stroke in form of Intraluminal middle cerebral artery occlusion (MCAO)
  • Determination of stroke-area due to an ischemic event in the brain
  • Assessment of the health-status of different regions of brain-slices by using TTC staining , grayscale-analysis and determination of ATP/MMP levels in dissociated brain cells

Projects: 

Publications:

Berressem D, Koch K, Franke N, Klein J, Eckert GP.
Intravenous treatment with a long-chain omega-3 lipid emulsion provides neuroprotection in a murine model of ischemic stroke - a pilot study 
PLoS ONE, 2016;11(11):e0167329. [open access]

Asseburg H, Schaefer C, Mueller M, Hagl S, Pohland M, Berressem D, Borchielline, M, Plank C, Eckert GP
Effects of grape skin extract on age-related mitochondrial dysfunction, memory and life span in C57BL/6J mice
NeuroMolecular Med, 2016, 18(3):378-95.

Hagl S, Berressem D, Bruns B, Sus N, Frank J, Eckert GP
Beneficial effects of ethanolic and hexanic rice bran extract on mitochondrial function in PC12 cells and the search for bioactive components 
Molecules 20(9), 16524-16539 [open access]

Hagl S, Berressem D, Grewal R, Grebenstein N, Frank J, Eckert GP
Rice bran extract improves mitochondrial dysfunction in brains of aged NMRI mice
Nutr Neurosci, 2015, accepted


Presentations at conferences:

Omega-3 Fatty Acids (Omegaven) protect from Mitochondrial Dysfunction in a MCAO mouse model of stroke“ Berressem D, Koch K, Klein J, Eckert GP "poster presentation at the annual meeting of the German Society for Pharmacy (DPhG), Frankfurt, Germany (2014).

Berressem D, Koch K, Klein J, Eckert GP "Omega-3 fatty acids (Omegaven) protect from mitochondrial dysfunction in a MCAO mouse model of stroke " 11th Biennial ISSFAL Congress, June 30, 2014, Stockholm, Sweden.

Attended congresses:
11th Biennial ISSFAL Congress, 2014 (Stockholm)
11th FACS meeting, 2013 (Bad Homburg)
Healthy ageing conference, 2012 (Frankfurt)



© Prof. Dr. Gunter P. Eckert 2017