Methods & Models

Fully equipped laboratories (140 square meter) are located in the Biomedical Research Center Seltersberg (BFS) Schubertstrasse 81, BFS, 35392 Giessen, Germany.

We use different in vitro and in vivo models to investigate the effects  of  food and bioactive food ingredients as well as pharmacological substances on physiological and pathological parameters reflecting the situation in the aging brain and in neurodegeneration. In cooperation with partners from nutritional science and medicine, we aim to evaluate our data in human studies. Standard analytical methods are used to determine the composition of food and food ingredients. Molecular, cellular and biochemical methods together with special techniques are used to characterize the biofunctional or pharmacological effects of test substrates.  

Models

Models invivo

in vitro 

Alzheimer:

  • SH-SY5Y cells
  • SY5Y-Mock cells
  • SY5Y-APP (695) wt cells
  • PC12neo cells
  • PC12sw cells

in vivo

aging:   

  • aged NMRI mice
  • aged C57/BL6 mice
  • SAMP8 and SAMR1 mice

Alzheimer‘s disease:

  • Thy1-APP751 SL mice

Lipid-reserach:

  • Dunkin Hartley guinea pigs

Ischemic Stroke:

  • MCAO model using CD-1 mice (in close cooperation with Dr. Jochen Klein)

Behaviour tests:

  • Open field
  • Passive avoidance
  • Social recognition
  • Object recognition
  • Y-maze
  • Rotarod
Nutritional Neuroscience opt.jpg


© Prof. Dr. Gunter P. Eckert 2017