Basic lab methods

Basic lab methods include:

qRT-PCR, gelelektrophoreses, Western blot, Radio-immunoassays, ELISA, HPLC-UV/Vis und HPLC-FD, DC, GC-MS, GC-FID, photometry, fluorescence spectroscopy and –microscopy, receptor binding assays, isolation and characterisation of synaptosomal and mitochondrial membranes, isolation and characterisation of lipid rafts, polarisation-fluorescence spectrometric measurement of membrane fluidity (DPH, TMA-DPH, Pyren), preparation of liposomes, determination of intracellular reactive oxygen species (FACS), malondialdehyde, hydroxynonenal, protein carbonyls, anti-oxidative enzymes (SOD, catalase, GPx, GR), hydroperoxides, 8-OHDG, isoprostanes.  

© Prof. Dr. Gunter P. Eckert 2018